DNA purification is among the most common and important methods in molecular biology. The purpose of DNA purification is to isolate the desired genetic material from the contaminant (proteins, cell membranes and RNA). This is a vital step in almost all molecular processes and must be done correctly in order to obtain top-quality, usable DNA.
There are several different methods for DNA purification. The selection is based on a variety of variables including the starting materials and downstream applications, cost and time limitations. Common DNA purification techniques include chemical treatment, enzymatic digestion, or mechanical destruction of cell samples or tissue followed by salting out of the proteins and precipitation of the DNA with ethanol.
Ethanol precipitation is an inexpensive, quick and simple method of desalting and concentrating DNA. DNA molecules form aggregates in the presence of monovalent cations like sodium, and then are filtered out of solution by large amounts of ethanol. This method is used to remove organic compounds, and other impurities. It is frequently utilized in conjunction with other purification methods.
Another method of DNA purification is anion exchange chromatography. The interaction between the negatively-charged DNA phosphate backbones and the positively charged surface molecules of resins bonds DNA in a solvent and positively charged https://mpsciences.com/2021/04/08/different-types-of-pcr-reagents/ resins. During the binding steps removal of contaminants is accomplished using a stringent wash process. The DNA that has been purified is eluted using low-salt conditions.